Τρίτη 21 Δεκεμβρίου 2021

Paracrine mechanisms of endothelial progenitor cells in vascular repair

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Via histochem

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Acta Histochem. 2021 Dec 17;124(1):151833. doi: 10.1016/j.acthis.2021.151833. Online ahead of print.

ABSTRACT

Endothelial progenitor cells (EPCs) play an important role in repairing damaged blood vessels and promoting neovascularization. However, the specific mechanism of EPCs promoting vascular repair is still unclear. Currently, there are two different views on the repair of damaged vessels by EPCs, one is that EPCs can directly differentiate into endothelial cells (ECs) a nd integrate into injured vessels, the other is that EPCs act on cells and blood vessels by releasing paracrine substances. But more evidence now supports the latter. Therefore, the paracrine mechanisms of EPCs are worth further study. This review describes the substances secreted by EPCs, some applications based on paracrine effects of EPCs, and the studies of paracrine mechanisms in cardiovascular diseases--all of these are to support the view that EPCs repair blood vessels through paracrine effects rather than integrating directly into damaged vessels.

PMID:34929523 | DOI:10.1016/j.acthis.2021.151833

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Reconstruction of midline abdominal defects with a deep inferior epigastric artery keystone-type perforator flap

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J Plast Reconstr Aesthet Surg. 2021 Dec 7:S1748-6815(21)00650-1. doi: 10.1016/j.bjps.2021.11.103. Online ahead of print.

NO ABSTRACT

PMID:34930700 | DOI:10.1016/j.bjps.2021.11.103

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Cerebrospinal Fluid Leak Detection with a Carbon Nanotube-Based Field-Effect Transistor Biosensing Platform

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ACS Appl Mater Interfaces. 2021 Dec 21. doi: 10.1021/acsami.1c19120. Online ahead of print.

ABSTRACT

Cerebrospinal fluid (CSF) leakage may lead to life-threatening complications if not detected promptly. However, gel electrophoresis, the gold-standard test for confirming CSF leakage by detecting beta2-transferrin (β2-Tf), requires 3-6 h and is labor-intensive. We developed a new β2-Tf detection platform for rapid identification of CSF leakage. The three-step design, which includes two steps of affinity chromatography and a rapid sensing step using a semiconductor-enriched single-walled carbon nanotube field-effect transistor (FET) sensor, circumvented the lack of selectivity that antitransferrin antibody exhibits for transferrin isoforms and markedly shortened the detection time. Furthermore, three different sensing configurations for the FET sensor were investigated for obtaining the optimal β2-Tf sensing results. Finally, body fluid (CSF and serum) tests employing our three-step strategy demonstrated high sensitivity, suggesting its potential to be used as a rapid diagnostic tool for CSF leakage.

PMID:34932323 | DOI:10.1021/acsami.1c19120

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