Conventional polymerase chain reaction and amplification refractory mutation system-multi-gene/ multi-primer PCR in the diagnosis of female genital tuberculosis

Abstract

This review mainly summarizes disease, immune-pathology and the clinical usefulness, advances, potential applications and limitations of new, cutting-edge technology (MG/MP-PCR and/or ARMS–MG/MP-PCR) in the detection of female genital tuberculosis (FGTB) disease and gene polymorphism among infertile patients. The investigation was set in the Department of Zoology, Osmania University and National Institute of Nutrition, Hyderabad, India. Desired articles were critically reviewed and analysed. Keywords and NET searches were conducted in all electronic databases starting from September, 2006. Full-text English-language reviews and research articles describing FGTB, infertility, gene polymorphism, conventional polymerase chain reaction (PCR) and multigene (MG)/multiprimer (MP)-PCR were included. The current review provides a comprehensive overview on the PCR and types (multiplex, nested, RT etc.) including the reagents, cycling conditions and pitfalls in the detection of FGTB disease and gene polymorphism among infertile patients. It provides limited information on MG/MP-PCR. At present, conventional PCR, MG/MP-PCR and/or amplification refractory mutation system (ARMS)–MG/MP-PCR have emerged as scientific innovations and perform significant function in medical research, mutational analysis and clinical investigations. This review admits that MG/MP-PCR and/or ARMS–MG/MP-PCR has the capacity to diagnose disease rapidly and to genotype a large number of samples. MG/MP-PCR and/or ARMS–MG/MP-PCR are considered as simple, reliable, non-isotopic, low-cost, fast, accurate and relatively easy-to-perform procedure. This review suggests that this method needs to be critically evaluated using huge number of clinical samples occurring across the world and then can be accredited for clinical utilization.

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