Κυριακή 25 Ιουνίου 2017

Effects of Acute Altered Gravity During Parabolic Flight and/or Vestibular Loss on Cell Proliferation in the Rat Dentate Gyrus.

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Effects of Acute Altered Gravity During Parabolic Flight and/or Vestibular Loss on Cell Proliferation in the Rat Dentate Gyrus.

Neurosci Lett. 2017 Jun 20;:

Authors: Zheng Y, Gliddon CM, Aitken P, Stiles L, Machado ML, Philoxene B, Denise P, Smith PF, Besnard S

Abstract
Both parabolic flight, i.e. a condition of altered gravity, and loss of vestibular function, have been suggested to affect spatial learning and memory, which is known to be influenced by neurogenesis in the hippocampus. In this study we investigated whether short alternated micro- and hyper-gravity stimulations during parabolic flight and/or loss of vestibular function, would alter cell proliferation in the hippocampal dentate gyrus of rats, by measuring the number of bromodeoxyuridine (BrdU)-incorporated cells. Rats were randomly allocated to the following experimental groups: (1) sham transtympanic saline injection only (n=5); (2) bilateral vestibular deafferentation (BVD) by sodium arsanilate transtympanic injection only (n=5); (3) sham treatment and parabolic flight (n=5); (4) BVD and parabolic flight (n=6). Forty-two days following transtympanic injection, the animals were subjected to parabolic flight in an awake restrained condition after habituation. A modified Airbus A300 aircraft was flown on a parabolic path, creating 20 secs of 1.8G during both climbing and descending and 22 secs of 0G at the apex of each parabola. The no flight animals were subjected to the same housing for the same duration. Immediately after the parabolic flight or control ground condition, animals were injected with BrdU (300mg/kg, i.p). Twenty-four hs after BrdU injection, rats were sacrificed. BrdU immunolabelling was performed and the number of BrdU(+ve) cells in the dentate gyrus of the hippocampus was quantified using a modified fractionator method. BVD caused a large and significant reduction in the number of BrdU-positive cells compared to sham animals (P≤0.0001); however, flight and all interactions were non-significant. These results indicate that BVD significantly decreased cell proliferation irrespective of the short exposure to altered/modified gravity.

PMID: 28645788 [PubMed - as supplied by publisher]



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