Evaluation of the Grafted Fascia in the Vocal Fold of Dogs: A Histologic Study

Publication date: Available online 23 March 2017
Source:Journal of Voice
Author(s): Eduardo G.B. Carvalho, Henrique F. Pauna, Almiro J. Machado, Ester M.D. Nicola, Albina M.A.M. Altemani, Agrício N. Crespo
IntroductionThere is no consensus on the ideal graft for medialization surgery of the vocal folds in the literature. One of the most favorable proposals is the use of autologous fascia, which seems limited by the lack of information regarding the integration of grafted tissue. Our study aims to evaluate the degree of fully engrafted fascia integration in the vocal fold lamina propria of dogs.Materials and MethodsFourteen adult mongrel dogs that underwent intravenous general anesthesia were selected and kept under spontaneous ventilation. A fascia lata fragment of 4 cm² was obtained from the right leg of each dog. The dogs underwent laryngoscopy; a 3 mm incision was made in the vocal process, next to the vestibular process, and the fascia was grafted into the right vocal fold. The left vocal fold was used as a control. The animals were divided into two groups: group A, evaluated after 2 months of the procedure, and group B, evaluated after 6 months of the procedure. Histologic analysis was made semiquantitatively regarding the presence of inflammatory reaction, fibrosis, and neovascularization.ResultsOur final studied group comprised 12 dogs. Microscopic examination of the larynx revealed the absence of any detectable inflammation in the incision site. The lamina propria of the grafted vocal fold showed identifiable compact, thick, and eosinophilic collagen bands. The surrounding tissue showed thin collagen bands with some organization, similar to the contralateral vocal fold.ConclusionThe grafted fascia integrates into the vocal fold lamina propria and seems not to cause inflammatory reaction response.



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